![]() We will create a foundational repository of isogenic-induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9-based genetic engineering. However, this requires readily available, disease-relevant cellular models of Alzheimer’s and related dementias and phenotypic datasets of the effects of gene mutations on cellular pathways, which are not currently available in the field. To develop effective treatments for Alzheimer’s and related dementias, we must identify how individual mutations impact cellular pathways and contribute to Alzheimer’s and related dementias pathology. Continue to develop approach as better technology emerges (e.g.Researchers have identified more than 50 regions of the genome (called loci) that contain variants that may increase the risk for the disease.On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010.Future enhancer cloning will be very simple and efficient.2 enhancer elements are ready for targeting into JM8A3 ES cells (this month).2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges).All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete.Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63-CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knock-in).Goal: To generate 15 Cre driver strains for orofacialclefting research.new ENU induced mutant strain (on FVB.A) įaceBaseCre Driver Project:Current progress and future plans.Bambi, Osr1, Osr2, Jag2, Snai1 and Snai2, Gad1, Slc32a1, Tgfbr2, Wnt9b. ![]() ![]() new knockout strains - most on B6 129 background.most are public, some still private, some imported and under development for distribution.~ 50 strains in the JAX FaceBase Repository.The Jackson LaboratoryBar Harbor, MEįaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution Genetic tools and resources for orofacialclefting research PIs: Leah Rae Donahue, Ph.D. ![]()
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